Our office uses state of the art sterilization to ensure patient safety. Sterilization and disinfection are the basic steps in instrument processing and surface asepsis. Sterilization refers to the use of a physical or chemical procedure to destroy all forms of microorganisms, including the highly resistant spores.
We use Rapid Steam Autoclave at 275º F(35psi), for 15-20 minutes
First, the instruments are prepared for the sterilization process. Patient debris and fluids are removed by placing the instruments in 3.2% glutaraldehyde for 40 minutes. Following this pre-disinfection step, the instruments are transferred to an ultrasonic cleaner for another 15 minutes. Then the instruments are rinsed, dried, placed in self-sealing sterilization pouches and sterilized in the autoclave. Instruments which can not be heat sterilized, are immersed in 2% glutaraldehyde for 10 hours to cold sterilize.
We use Biological, Chemical and Mechanical indicators to monitor our sterilization process.
Using bacterial spores to monitor the sterilization process is referred to as biologic monitoring (or spore-testing), and the bacterial spores used for monitoring the sterilization process are referred to as biologic indicators (BIs). Of the three methods, biologic monitoring is regarded as the most valid for monitoring the sterilization process, for it uses live, highly resistant bacterial spores.
We biologically monitor our sterilizer once a week to ensure complete sterilization using spore strips and keep accurate records for our monitoring. These strips are enclosed in a glassine envelope and processed through the sterilizer. They are then sent to our spore testing center where they are tested for live spores.
Chemical monitoring involves using chemical indicators (CIs) that change color or form when exposed to specific high temperatures or to the sterilizing conditions within a sterilizer. This is referred to as chemical monitoring (or process monitoring). We use sterilization pouches that have a special marking that changes color when subjected to sterilizing temperatures.
Mechanical monitoring involves observing and recording the physical aspects (e.g., temperature, pressure or time) of the cycle when the sterilizer is being operated. Our Sterilizer is serviced regularly to ensure proper functioning.